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The Training Course in The Super Mario Bros. Movie is a floating obstacle course that Mario goes through train to fight against Bowser. When Mario and Princess Peach arrive at the location, a giant Question Mark Block appears out of the water, and turns into the training course.

It features various blocks and objects from the Mario franchise, as well as dummy versions of enemies. Peach tells Mario that if he manages to finish the course, he can join her on her journey, and demonstrates the course by going through it herself, completing it perfectly. Mario is seen attempting the course during the day and night while Peach watches and failing multiple times in different ways, including being knocked out by a dummy Fire Bar, being blasted by a dummy Bullet Bill, and falling after standing on a Donut Block for too long. The Training Course later reappears during the end credits.

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